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AHG must be added immediately after washing to prevent the possibility of a false negative;...

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AHG must be added immediately after washing to prevent the possibility of a false negative; a false negative can occur in this situation because previously bound globulins may dissociate from red cells, leaving insufficient antibody coating on the red cells to produce a reaction, or free antibody may directly neutralize antiglobulin reagent.

Blood bank

A false-negative reaction while performing the DAT technique may be the result of:

A.

Red cell/AHG tube is over centrifuged

B.

Blood collected in tube containing silicon gel

C.

Saline used for wash stored in glass or metal container

D.

AHG addition delayed for 40 or more minutes

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